12/23/2023 0 Comments P5 mouse![]() ![]() Furthermore, in this study, a self-organizing map (SOM) revealed that plasma amino acid concentrations depended on age and different protein diets, and were associated with hepatic lipid content in young and middle-aged male mice which were fed diets containing different protein contents.Īnimal experiments were conducted in accordance with animal care and protocols approved by the Institutional Animal Care and Use Committee of the Tokyo Metropolitan Institute of Gerontology (TMIG, Tokyo, Japan) (Permit Number:17059) and the Guidelines for the Care and Use of Laboratory Animals of TMIG. To determine the same, we fed young and middle-aged male mice diets containing different protein contents and investigated the weight dynamics of individual skeletal muscles, such as the gastrocnemius (Gas), tibialis anterior (TA), plantaris (Pla), extensor digitorum longus (EDL), soleus (Sol), lipid profile in liver and plasma, and amino acid composition in plasma. However, the amount of protein that should be consumed to maintain metabolic health while approaching old age is still unclear. showed that sex and genetic background are key factors in the response to protein restriction using a multi-omics approach. reported that late-life dietary restriction, the switch from dietary restriction to ad libitum at 24 months of age, increased mortality in female mice, and the switch from ad libitum to dietary restriction slightly increased survival, which considers nutritional memory. In addition, more recent studies on dietary macronutrients and aging have focused on nutritional memory, sex, and the genetic background of mice. showed the possibility of minimizing age-specific mortality throughout life by changing the ratio of dietary protein to carbohydrate during approach to old age in mice. Therefore, the optimal balance of macronutrients for ideal health outcomes may vary across different life stages. ![]() In contrast, adequate dietary protein intake is recommended to prevent frailty and sarcopenia in humans. have reported that the ratio of dietary macronutrients (protein, carbohydrate, and fat), and not caloric intake, dictate cardiometabolic health and aging, and also that low-protein and high-carbohydrate diets are associated with the longest lifespan in lifelong ad libitum-fed mice. Recently, researchers have found a plausible way to vary the ratio of dietary macronutrients, such as proteins, carbohydrates, and fat in rodents. However, the feasibility of these interventions in humans is problematic. Nutritional interventions, such as calorie and protein restrictions and intermittent fasting, are known to increase the healthspan and lifespan of primates and rodents. In particular, the prevention and improvement of emerging diseases such as sarcopenia, frailty, and non-alcoholic fatty liver disease (NAFLD), are important issues to be addressed in this context. Therefore, various nutritional and pharmacological methodologies have been proposed to delay the aging process and prevent age-related diseases such as cancer, dementia, metabolic syndrome, diabetes mellitus, and cardiovascular diseases. ![]() Lifespan is defined as “how long one lives” and healthspan as “the healthy periods, without diseases, of one’s life”. In recent years, the extending of health span has been accepted as a goal of research on aging in many countries, where the number of elderly people is increasing. ![]() Results indicate that the moderate protein intake percentages (25% and 35%) are required for maintaining metabolic health in middle-aged mice, which is similar to that in young mice. Self-organizing map (SOM) analysis revealed that plasma amino acid profiles differed depending on age and difference in protein diet and were associated with hepatic triglyceride and cholesterol levels. The low-protein diet developed mild fatty liver, with middle-aged mice showing more lipids than young mice, whereas the moderate-protein diet suppressed lipid contents and lowered the levels of blood glucose and lipids. To investigate the appropriate ratio of nutrients, particularly proteins, for maintaining metabolic health while approaching old age, young (6-month-old) and middle-aged (16-month-old) mice were fed isocaloric diets with varying protein percentages (5%, 15%, 25%, 35%, and 45% by calorie ratio) for two months. Nutritional requirements for maintaining metabolic health may vary with each life stage, such as young, middle, and old age. ![]()
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